The offspring are heterozygous for the floxed target gene (C) and breed with the homozygous floxed mouse (B). The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This ...

In addition, loxP-flanked target genes can differ dramatically with respect to their sensitivity to Cre-mediated recombination. Thus, to be sure about the specificity of a given conditional ...