细胞实验方面，他们培养了 Huh7 和 HepG2 两种肝癌细胞系，采用细胞活力检测（如 CCK-8 法）、克隆形成实验、划痕愈合实验、Transwell 迁移和侵袭实验等，来观察褪黑素对癌细胞增殖、迁移和侵袭能力的影响；通过蛋白质免疫印迹法（Western blot）和荧光定量聚合酶 ...

F-F’. Protein levels of pAKT (Ser473) and total AKT in eWAT of ND mice or DIO mice treated with vehicle or STF, analyzed with Western blotting (F). GAPDH as a loading control. Fasted mice were IP ...