This revision of important work is a versatile addition to the chemical protein modifications and bioconjugation toolbox in synthetic biology. The technology developed cleverly uses Connectase to ...

Darnell discovered that when they put this exclusively human variant of NOVA1—an RNA-binding protein in the brain known to be crucial to neural development—into mice, it altered their ...

The column with bound proteins was washed with 2× column volume (cv) of the same lysis buffer. Bound protein was subsequently eluted using a linear gradient concentration of imidazole (20–500 mM, 20 × ...