Annexin A5 - Wikipedia
Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane.
Annexin V Staining | Thermo Fisher Scientific - US
Learn how to use annexin V conjugates to detect apoptotic cells by flow cytometry or imaging. Find selection guide, protocols, and kits for different annexin V conjugates.
Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining ...
This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis.
Because the anticoagulant protein Annexin V binds with high affinity to phosphatidylserine, fluorochrome-conjugated Annexin V can been used to detect apoptotic cells by flow cytometry. Annexin V is commercially available, conjugated to most common fluorochromes.
Expression, purification and use of recombinant annexin V for the ...
2009年9月3日 · Measurement of plasma membrane phosphatidylserine externalization, using fluorescently labeled annexin V, is widely used for the detection of apoptotic cells. Here we describe protocols for...
Imaging the Molecular Signatures of Apoptosis and Injury with ...
Radiolabeled annexin V is one of the few radiotracers that has been widely used in Phase II trials in NSCLC and is still under development. In this article we will first outline the signaling pathways of apoptotic cell death that form the basis of radiolabeled annexin V imaging.
Annexin V staining assay protocol for apoptosis - Abcam
2021年11月10日 · Discover the procedure for early detection of apoptosis using annexin V staining, and propidium iodide (PI), in our detailed step-by-step protocol.
Quantitation of Apoptosis and Necrosis by Annexin V Binding ... - PubMed
2016年11月1日 · Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane.
BD Pharmingen™ FITC Annexin V Apoptosis Detection Kit I - BD …
FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids.
Annexin-V stabilizes membrane defects by inducing lipid phase ... - Nature
2020年1月13日 · Annexins are abundant cytoplasmic proteins, which bind to membranes that expose negatively charged phospholipids in a Ca 2+ -dependent manner. During cell injuries, the entry of extracellular Ca...