Structural evidence for extracellular silica formation by diatoms …
2021年7月30日 · Here, we study the formation of long silicified extensions that characterize many diatom species. We use cryo-electron tomography to image silica formation in situ, in 3D, and at a...
Exocytosis of the silicified cell wall of diatoms involves ... - Nature
2023年1月30日 · Here we study the membrane dynamics during cell wall formation and exocytosis in two model diatom species, using live-cell confocal microscopy, transmission electron microscopy and...
Understanding Diatom Cell Wall Silicification—Moving Forward
2018年4月10日 · The silicified cell walls of diatoms have inspired the interest of researchers for several centuries, and our understanding of their properties and formation has developed in synch with the development of observational and analytical techniques.
Cell Wall Formation in Diatoms: Morphogenesis and Biochemistry …
Cell Wall Formation in Diatoms: Morphogenesis and Biochemistry. In: Simpson, T.L., Volcani, B.E. (eds) Silicon and Siliceous Structures in Biological Systems. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5944-2_7
Intracellular morphogenesis of diatom silica is guided by local ...
2024年9月10日 · We propose that silica growth and morphogenesis result from the biophysical properties of the SDV and plasma membranes. The hallmark of the silica cell walls of diatoms is their...
Identification of Proteins from a Cell Wall Fraction of the Diatom ...
2006年1月1日 · Diatoms are unicellular eucaryotic algae with cell walls containing silica, intricately and ornately structured on the nanometer scale. Overall silica structure is formed by expansion and molding of the membrane-bound silica deposition vesicle.
Diatom Cell Wall Proteins and the Cell Biology of Silica ...
1998年9月1日 · Diatoms are unicellular algae that are well-known for the spectacular design of their cell walls. The diatom cell wall is made of amorphous, hydrated silici-umdioxide (silica) which is associated with proteins and polysaccharides. It is the structure and arrangement of the silica scaffold that gives rise to the intricate patterns of the cell wall.
Diatoms—From Cell Wall Biogenesis to Nanotechnology
Diatoms are single-celled algae that produce intricately structured cell walls made of nanopatterned silica (SiO 2). The cell wall structure is a species-specific characteristic demonstrating that diatom silica morphogenesis is genetically encoded.
Comparative Gene Analysis Focused on Silica Cell Wall Formation ...
2020年6月3日 · In this study, we assembled de novo transcriptomes of three diatom species, Nitzschia palea, Achnanthes kuwaitensis, and Pseudoleyanella lunata, and compared protein-coding genes of five genome-sequenced diatom species. These analyses revealed a number of diatom-specific genes that encode putative endoplasmic reticulum-targeting proteins.
New structural insights into the cell-wall polysaccharide of the diatom …
2017年9月1日 · The main polysaccharide, a sulphated glucuronomannan, has been observed in many diatoms suggesting that it plays an important role in cell-wall biogenesis. We extracted and purified the polysaccharide, prepared oligosaccharide series and analysed the structure of poly- and oligosaccharides using chromatography and NMR spectroscopy methods.